|Surname, First name
|Project 1: Advanced Imaging of Cancer Metastasis in Whole Mouse Body / Project 2: Whole-body imaging of nanoparticles
|We have previously established a novel primary microglia culture protocol from mouse retina, which allows for good reproducibility, high cell numbers and long in vitro viability. In order to be able to efficiently manipulate retinal microglia in vivo and in vitro, efficient gene delivery technologies are needed. Within this project, we will evaluate novel-engineered adeno-associated virus (AAV) capsids regarding efficacy and specificity. These AAV variants will be tested in our mouse retinal microglia culture model, mouse retinal explant cultures and in a mouse model of retinal degeneration to validate their efficacy and specificity in vitro, ex vivo and in vivo.
|The Misgeld lab uses in vivo imaging methods (ranging from wide-field time-lapse to two-photon microscopy) to study the development and degeneration of neurons and their processes.
|The main interest of the laboratory is to study the role of cerebral vessels for the pathophysiology of acute and chronic brain injury and to use the evolving knowledge for the development of novel therapeutic strategies for patients. For this purpose we use clinically relevant mouse models for acute and chronic brain injury and investigate neuro-vascular morphology and function by in vivo microscopy using conventional and 2-photon fluorescence microscopy.
|On a systems neuroscience level we are working on the brain mechanisms of pain perception. We aim to further the understanding of the brain processes, which determine the individual sensitivity of a person to pain, which explain why we perceive the same painful event differently in different situations.
|The Amgen scholar will be involved in designing (cloning) and optimizing (purification) different protein constructs followed by screening LLPS conditions using microscopy and biochemical characterization, ie protein-protein interactions, using nuclear magnetic resonance (NMR) spectroscopy, electron microscopy (EM) and other biophysical techniques (ITC, SLS), which will ultimately provide foundation to obtain a structural model of the peroxisomal protein import machinery.
|Schmidt, Mathias V.
|Project 1: Characterizing long-term outcomes of developmental stress exposure in a translational mouse model / Project 2: Investigation into the Role of FKBP51 in Obesity and Type 2 Diabetes
|We aim to identify circulating signatures that inform on the local and systemic effects of stroke and to explore the underlying molecular and pathophysiological mechanisms. Events in most organs including the local and systemic events (e.g. stress) related to acute stroke are captured by the circulating proteome and metabolome.
|The biochemical pathway of chlorophyll degradation has only recently been characterized. The metabolites that are yielded from the breakdown path – the phyllobilins – were shown to have antioxidant properties.
|Novel molecular therapeutics like siRNA, microRNAs or antagomiRs require potent extracellular and intracellular delivery devices to be effective antitumor agents.
|The Laboratory for Neurodegenerative Disease Research focuses on the generation of Amyloid ß-peptide (Aß) as the major constituent of neurotoxic amyloid plaques in Alzheimer's Disease (AD).