Affiliation
Institute of Medical Psychology and Biomedical Center (BMC), LMU Munich
Contact
Goethestrasse 31
80336 Munich
Email:
crobles@med.lmu.de
Website:
https://www.med.lmu.de/imp/en/research-groups/robles-lab-sytems-chronobiology/
Research Focus
Project overview:
Circadian clocks are cell endogenous, self-sustainable oscillators present in virtually every cell in the body, playing a fundamental role in cellular and tissue physiology. This internal timing system enables our organs to molecularly anticipate and prepare their metabolism for daily recurrent needs associated with environmental changes. While the circadian clock regulates metabolism, metabolic states, in turn, provide feedback to the circadian clock, modulating its function. We are investigating this molecular crosstalk in peripheral metabolic tissues from mice, employing interaction and spatial proteomics as well as phosphoproteomics.
Our prelimmary work has identified tissue specific complex constituents of the core clock transcriptional heterodimer complex at the chormatin. We are now functionally validating those proteins in mouse tissues as well as in culture cell lines using a variety of methods: iRNA knowdown, CRIRSP/Cas9 deletion, interaction proteomics and real time bioluminescence.
Project supervisor: Fatih Aygenli (Robles Lab)
Must-have lab skills: Cell culture, transfection/electroporation, plasmid isolation, cloning.
Nice-to-have: proteomics and bioinformatic skills.