MCN Junior Research Group & Group Epigenetic Engineering
Helmholtz Zentrum, German Research Center for Environmental Health
MCN Junior Research Group, Munich Center for Neurosciences
LMU Munich, BioMedical Center
Grosshaderner Strasse 9
The research aim of the lab is to investigate which of the myriad of epigenomic marks have significant functional relevance in mediating cell identity or disease phenotypes.
- Which epigenetic mechanisms trigger these phenotypes?
- Where are causal epigenomic marks located and how do they work?
Primary techniques and models: To address these questions we use CRISPR/dCas9 fusion proteins to manipulate epigenomic features and/or gene expression. For this we develop, modify and use the following tools and models:
- Cellular reprogramming, differentiation and trans-differentiation
- Epigenome and transcriptome editing
- gRNA multiplexing, libraries and epigenomic screens
Image: Schematic of dCas9-based epigenome editing approaches. Taken from “Entering the post-epigenomic age: back to epigenetics.” By Bultmann S, Stricker SH. Open Biol. 2018 Mar;8(3). pii: 180013. doi: 10.1098/rsob.180013. PMID: 29593118
More information can be found in our previous publications:
- Stricker et al., 2017 Nature reviews genetics
- Koferle et al., 2017 BMC Genomics
- reunig et al., 2018 Plos ONE
Model Organism: mouse/human stem cells