LMU Medical Faculty, Adolf Butenandt Institute, Department of Molecular Biology, Chromatin Dynamics and Nuclear Function
Biomedical Center Munich
Großhaderner Strasse 9
+49 (89) 2180 - 75428
Fax: +49 (89) 2180 - 75425
Research in Prof. Becker's lab focuses on the molecular mechanisms, by which dedicated modulators of chromatin structure regulate the activity of chromosomal loci. Their aim is to understand the principles that allow the targeting of regulators to the specific sites in chromatin, the nature of the structural changes provoked by these factors, as well as the molecular mechanisms through which alterations of chromatin structure affect enzymes involved in nucleic acid metabolism. The research methods include biochemistry, cell biology, genetics and molecular biology.
Biochemistry: Analysis of protein interactions with DNA, RNA, other proteins, nucleosomes. Purification of regulatory ribonucleoprotein complexes from fly tissue. Reconstitution of nucleosomes and chromatin fibres for interaction studies.
Cell biology: Immunofluorescence microscopy of developmental stages of Drosophila.
Genetics: Classical fly genetics; cell-type-specific RNA interference and overexpression of regulatory proteins.
Molecular biology: cloning and expression of mutated proteins using the Gateway system.
Model Organism(s): Drosophila melanogaster and tissue culture cells